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无壁孔板是如何工作的?

DropArray孔板是由疏水表面和96个或384个亲水点组成,以标准SBS / ANSI格式排列。 DropArray孔板设计独特,主要有三种应用

  • Laminar Wash™ 孔板
    层流洗涤板应用于流式细胞分析中悬浮细胞的处理过程。每一块孔板有96个孔,每个孔带有两个附属小孔,以允许通过DA-Cell清洗站(HT1000)进行双喷嘴层流洗涤。每个孔都有单独的喷嘴,所有孔同时进行快速洗涤。每个孔体积可达到70 ul,相当于大约500万个细胞。
  • Laminar Wash™ 孔板
    DA-Bead板用于基于珠的免疫分析。将板格式化为96孔或384孔格式,然后通过DropArray清洗站(LT210或HT200)用缓冲液充满板,进行层流清洗。
DropArray技术的应用
有关DropArray的发表文章

Le Burel S, Thépenier C, Boutin L, lataillade j-j, Peltzer J. Effect of Mesenchymal Stromal Cells on T cells in septic context: immuno-suppression or immuno-stimulation? Stem Cells and Development. 2017.

Wei J, Versteeg L, Liu Z, et al. Yeast-expressed recombinant As16 protects mice against Ascaris suum infection through induction of a Th2-skewed immune response. PLOS Neglected Tropical Diseases. 2017;11(7):e0005769.

Champion TC, Partridge LJ, Ong S-M, Malleret B, Wong S-C, Monk PN. Monocyte Subsets Have Distinct Patterns of Tetraspanin Expression and Different Capacities to Form Multinucleate Giant Cells. Frontiers in Immunology. 2018;9:1247.

Weatherhead JE, Porter P, Coffey A, et al. Ascaris Larval Infection and Lung Invasion Directly Induces Severe Allergic Airway Disease in Mice. Infection and Immunity. 2018.

Munakata S, Ishimori K, Kitamura N, Ishikawa S, Takanami Y, Ito S. Oxidative stress responses in human bronchial epithelial cells exposed to cigarette smoke and vapor from tobacco- and nicotine-containing products. Regulatory Toxicology and Pharmacology. 2018.

Briggs N, Wei J, Versteeg L, et al. Trichuris muris whey acidic protein induces type 2 protective immunity against whipworm. PLOS Pathogens. 2018;14(8):e1007273.

Akiyama H, Miller CM, Ettinger CR, Belkina AC, Snyder-Cappione JE, Gummuluru S. HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction. Nature Communications. 2018;9(1):3450.

Denis GV, Sebastiani P, Andrieu G, et al. Relationships Among Obesity, Type 2 Diabetes, and Plasma Cytokines in African American Women. Obesity. 2017:1916-1920.

Denis GV, Sebastiani P, Bertrand KA, et al. Inflammatory signatures distinguish metabolic health in African American women with obesity. PLOS ONE. 2018;13(5):e0196755.

Francica BJ, Ghasemzadeh A, Desbien AL, et al. TNFα and Radioresistant Stromal Cells Are Essential for Therapeutic Efficacy of Cyclic Dinucleotide STING Agonists in Nonimmunogenic Tumors. Cancer Immunology Research. 2018;6(4):422.

Jones K, Versteeg L, Damania A, et al. Vaccine-Linked Chemotherapy Improves Benznidazole Efficacy for Acute Chagas Disease. Infection and Immunity. 2018;86(4).

Ong S-M, Hadadi E, Dang T-M, et al. The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence. Cell Death & Disease. 2018;9(3):266.

Versteeg L, Le Guezennec X, Zhan B, et al. Transferring Luminex® cytokine assays to a wall-less plate technology: Validation and comparison study with plasma and cell culture supernatants. Journal of Immunological Methods. 2017;440:74-82.

Tan-Garcia A, Wai L-E, Zheng D, et al. Intrahepatic CD206+ macrophages contribute to inflammation in advanced viral-related liver disease. Journal of Hepatology. 2017.

See P, Dutertre C-A, Chen J, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356(6342).

McGovern N, Shin A, Low G, et al. Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2. Nature. 2017;advance online publication.

Lim JS, Ibaseta A, Fischer MM, et al. Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer. Nature. 2017;545(7654):360-364.

Jing W, Cui J, Fu Y, et al. A novel method for multiple cytokines/chemokines DropArray assay in 5 μL of bronchoalveolar lavage fluid (BALF) in mice. Sensors and Actuators B: Chemical. 2017;242:195-201.

Durairaj RRP, Aberkane A, Polanski L, et al. Deregulation of the endometrial stromal cell secretome precedes embryo implantation failure. Molecular Human Reproduction. 2017.

Le Guezennec X, Lye M. Improving Bead-Based Multiplexing in Bodily Fluids. Genetic Engineering & Biotechnology News. 2016;36(2):12-13.

Jani D, Allinson J, Berisha F, et al. Recommendations for Use and Fit-for-Purpose Validation of Biomarker Multiplex Ligand Binding Assays in Drug Development. The AAPS Journal. 2016;18(1):1-14.

Du G, Fang Q, den Toonder JMJ. Microfluidics for cell-based high throughput screening platforms—A review. Analytica Chimica Acta. 2016;903:36-50.

Le Guezennec X, Quah J, Tong L, Kim N. Human tear analysis with miniaturized multiplex cytokine assay on “wall-less” 96-well plate. Molecular Vision. 2015;21:1151-1161.

Ingle GS, Scales SJ. DropArray™, a Wall-Less 96-Well Plate for Uptake and Immunofluorescence Microscopy, Confirms CD22 Recycles. Traffic. 2014;15(3):255-272.

Dreze M, Calkins AS, Gálicza J, et al. Monitoring Repair of UV-Induced 6-4-Photoproducts with a Purified DDB2 Protein Complex. PLOS ONE. 2014;9(1):e85896.

Quiñones GA, Moore TI, Nicholes K, et al. Application of a new wall-less plate technology to complex multistep cell-based investigations using suspension cells. Blood. 2013;121(7):e25.

Kozak KR, Wang J, Lye M, et al. Micro-volume wall-less immunoassays using patterned planar plates. Lab on a Chip. 2013;13(7):1342-1350.


数据质量最高化,试剂消耗最小化。
DropArray技术在三个主要研究领域中提供应用和特定产品:

磁珠免疫分析

流式细胞应用中细胞的染色及悬浮细胞的保留

高内涵筛选应用,包括转染,自噬和药物筛选

应用 传统困难 DropArray解决方案 DropArray平台优势
流式细胞分析中无离心细胞处理 传统的基于离心的细胞制备耗时、费力、浪费昂贵的试剂,还会对细胞产生压力。细胞的大量丢失、洗涤不彻底,都会为圈门及细胞群分辨造成困难。 DA-Cell 层流洗涤技术有高于95%的细胞保留率,可在2-4分钟内完成洗涤,多色实验中也更容易进行细胞群圈门及分辨,从而提供一致性、不依赖于操作者、可信的数据。
磁珠免疫分析 传统的微孔板需要大量试剂。 磁珠和测定试剂价格昂贵,限制了数据产出。大部分昂贵的磁珠被浪费掉。板壁限制了有效地洗涤,并使磁珠聚集影响了数据质量。 DA-Bead DA-Bead板的小型化将样品体积和磁珠消耗量降低了5倍,并将测定成本降低了60%以上。 层流洗涤效率高,并且防止磁了珠的聚集。
实验板的独特设计

应用 研究领域 规格 层流洗涤方法 体积
层流洗涤 流式细胞术悬浮细胞准备 细胞分析 96孔 双喷嘴单细胞 最高70ul
DA-Bead 微球分析小型化 蛋白质检测/免疫测定 96孔和384孔 全板洗涤 96孔5-20ul
384孔2-4ul