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即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化

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应用COVID-19和传染病
COVID-19和传染病
通过减少传染性样本气溶胶的产生,来提高生物安全性。
概述
升级您处理细胞的方式以获得
可靠一致的数据。
Table 1. Risk prioritization of selected routine laboratory tasks
Task or activityExposure risk


Potential hazardLikelihoodConsequenceRisk rating
Subculturing blood culture bottleNeedle stick — percutaneous inoculationLikelyInfection; medical treatmentHigh
Aerosols — inhalationModerateInfection; medical treatmentMedium
Splash — direct contact with mucous membranesModerateInfection; medical treatmentHigh
CentrifugationAerosols — inhalationLikelyInfection; medical treatmentHigh
Performing Gram stainAerosols from flaming slidesModerateColonization; infectionModerate
Preparing AFB smear onlyAerosols from sputum or slide preparationLikelyIllness; medical treatment; diseaseHigh
Performing catalase testingAerosols — mucous membrane exposureUnlikelyColonization; infectionLow
AFB culture work-upAerosols — inhalationLikelyIllness; medical treatment; diseaseHigh
Abbreviation: AFB = acid-fast bacillus.

世界卫生组织实验室关于新型冠状病毒SARS-COV-2的研究指南指出,“所有技术程序都应以最大限度减少气溶胶和飞沫产生的方式进行。”

Curiox Biosystems引入了一种全新的、更安全的方法,用于在没有离心机的情况下制备潜在或已知感染性血液样本以进行免疫细胞分析,消除潜在感染性样本的物化,该方法结合了Laminar Wash HT2000系统的新配置,该系统与STEMCELL Technologies EasySep RBC去除试剂包装在一起,能够在生物安全柜内从头到尾进行血液样本处理。

这种自然而温和的细胞制备方法依赖于层流洗涤技术,可提高已知在对抗COVID-19感染的免疫反应中起关键作用的稀有和敏感免疫细胞亚群(如单核细胞和中性粒细胞)的存活率、保留率和染色分辨率。即使对于依赖重复离心步骤的红细胞裂解等苛刻方法导致的脆弱细胞,由于压力降低而导致的数据质量改善也是显而易见的。

“The Laminar Washer is safer than flicking [minimizing exposure to infectious materials] and requires less space than centrifuges.”

Blanca Ponce-Ngo,Montefiore医疗中心

技术
传统离心 VS 层流洗涤技术

用于制备用于流式细胞术分析的单细胞悬液的免疫染色工作流程的比较。层流洗涤技术在洗涤方面同样有效,但无需使用离心机。

Melvin_in_lab-1-370x241.jpg
优势

用于单细胞测序和蛋白质组学的层流洗涤™技术

Laminar Wash HT2000和Laminar Wash MINI系统紧凑的外形设计可以轻松放置在生物安全柜中。层流洗涤™技术可以通过一下方式升级传染病样品制备:

通过生物安全柜,提高工作流程的安全性

减少样品产生的气溶胶

减少碎片、背景和仪器堵塞

提高分辨率和染色指数

特色资源
使用Laminar Wash™技术进行流式细胞术的免离心红细胞裂解和全血免疫染色
科研数据
使用层流洗涤™系统的研究数据
Laminar Wash对中性粒细胞和单核细胞均非常温和

在使用 100 μL 全人血的 16 色免疫分析面板中,将层流洗涤技术与离心方案进行了比较。与多个离心步骤相比,使用层流洗涤的一步洗涤最大限度地减少了细胞扰动,增加了单核细胞和中性粒细胞群的保留。

细胞碎片去除能力更强,细胞保留率更高

在荷瘤组织中进行细胞解离时,组织碎片和酶促碎片是影响肿瘤浸润淋巴细胞 (TIL) 恢复的主要因素。对于更具侵入性和免疫学挑战性的模型,如 B16F10 接种小鼠,有限数量的 TIL 的恢复阻碍了准确表征。在这里,将层流清洗技术与制备含有约 1,000,000 个肿瘤浸润细胞的未受攻击的小鼠样本的常规方法进行了比较。层流洗涤提高了活细胞计数并减少了碎片形成。与离心机的 10.5% 相比,更清洁的样品制备将淋巴细胞回收率提高了 90.6%。

数据改编自Curiox Biosystems 网络研讨会,Christoph S. Eberle 博士,Discovery Research Services at查尔斯河实验室,马萨诸塞州伍斯特

开始
自动化您的流式细胞术工作流程
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