即将举行的网络研讨会：实现无需离心的流式细胞术样品制备工作流程的标准化
来自小型化的可靠数据
众所周知，基于磁珠的测定有时会与血浆、血液和其他具有挑战性的生物样品发生磁珠聚集，这会影响蛋白质的可检测性并影响数据质量。科学研究表明，DropArray可以减少磁珠聚集，并且更好地读取磁珠，单独报告目标蛋白质的信号。
"...the DA-Bead method for analysis was comparable to, if not superior to, the conventional method in terms of consistency/precision, accuracy, sensitivity and dynamic range and these results are not specific to sample type, reagents, or commercial vendor."
Leroy Versteeg，贝勒医学院
CURIOX DA-Bead板 VS 传统孔板
A) 传统板（左）和 DA-Bead 板（右）中的孵育方法原理。 珠子在传统孔中以 75 µl 体积自由浮动，而珠子则通过孔下方的磁铁固定在 DA-Bead Plate 中的 15 µl 液滴中并摇动。
(B) 传统板（左）和 DA-Bead 板（右）中的洗涤方法原理。 带有传统板的磁体阵列要么在传统孔的侧面超级包装珠子，要么将珠子保持为单层，导致珠子吸入和/或使用基于喷嘴/移液器的分配器进行不完全清洗。相反，DA-Bead Plate 磁体配置将珠子保持为单层（右），从而以温和的层流和最小的残留洗涤缓冲液进行完全洗涤。
5倍的小型化将总分析成本降低了60-80%
DropArray™板可以在单个检测试剂盒中检测5倍以上的样本
偏最小二乘回归 (PLSR) 模型将 (A) 卵巢子宫内膜异位症与腹膜子宫内膜异位症、(B) 卵巢子宫内膜异位症与深层浸润性子宫内膜异位症以及 (C) 腹膜子宫内膜异位症与深层浸润性子宫内膜异位症分开。
主成分 (PC) 评分显示使用 PF 细胞因子可以很好地分离子宫内膜异位症亚型。(D-F)。
相应的 PLSR 系数分析揭示了描绘各种亚表型的细胞因子特征。
与特定子宫内膜异位症亚型（OE、PE 或 DIE）相关的细胞因子相对于其对照物的升高出现在图的相同上半部分或下半部分。
用 Alhydrogel、MPL 和 AddaVax 配制的 rAs37 免疫 BALB/c 小鼠的细胞因子谱。在用 rAs37 (25 μg/ml) 重新刺激 48 小时后，测定脾细胞上清液中的细胞因子水平。从相应 rAs37 再刺激样品的细胞因子值中减去未刺激样品中测量的细胞因子值。数据表示
为每组 (n = 5) 的平均值和单个值。*p<0.05，**p<0.01。
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