即将举行的网络研讨会：实现无需离心的流式细胞术样品制备工作流程的标准化
常见问题
一般常见问题
- Laminar Wash™ 板和传统微孔板之间的主要区别是什么？
- 层流洗涤板是平底无壁的，带有双卫星，其中孔是疏水的。当液体分配到孔中时，它会形成液滴，这些液滴由于疏水环境产生的表面张力而保持在固定位置。
- 细胞如何留在孔中而不被双喷嘴作用产生的层流冲走？
- During incubation, the cells settle to the bottom of the well without any physical attachment. Once the plate is loaded in the washer, two separate nozzles are lower towards the satellites in each well – one that dispenses buffer and the other one that aspirates. The laminar velocity is high at the top of the well, whereas the velocity at the bottom, where the cells are located, is close to zero.
- 使用层流洗涤板是否需要特殊技能，因为它没有墙？
- Using the Laminar Wash plates is easy and it does not require any special skills, plus they the plates are compatible with robotic arms.
- 在层流清洗机上清洗板需要多少清洗缓冲液和清洗时间？
- The HT2000 Washer requires ~200 ml for priming the MINI1000 Washer ~50 ml. Both washers use ~65 ml for a 1x washing cycle, and a typical 12x wash cycle needs ~4 minutes when set to the lowest speed (5 µl/s).
用于流式细胞术的层流洗涤
- 培养期间细胞沉降所需的最短时间是多少？
- The minimum time for optimized cell recovery is recommended at 15 minutes. The addition of 15 to 25 minutes of incubation time reduces the loss of cells.
- 我们可以使用层流洗涤板在带有 96 孔装载器的流式细胞仪中直接采集吗？
- Yes. The Laminar Wash plates are compatible with most flow cytometers with 96-well loaders.
- 我们对稀有细胞和事件感兴趣，通常需要更大的体积。层流洗涤板每孔的最大细胞数和试剂体积是多少？
- In the Laminar Wash plates you can process up to 10 million cells per well, and each well can hold up to 70 µl of reagent, and this volume can increase to 150 µl by using a simple accessory.
- 我们可以在 Laminar Wash 板上进行固定、透化和细胞内染色吗？
- Yes, per your usual protocol the fixing, permeabilization, and staining can be performed all on the Laminar Wash plate.
- 我需要运行一个完整的板还是可以运行部分板或重复使用该板？
- The plate can be run in full or partially. If only a few wells are used, the plate can be stored at 4°C for up to 3 weeks, and the remainder of the wells can be used later. However, wells cannot be reused and caution must be taken to document the wells that have been used.
- 一个孔的典型体积是多少？它可以包含多少个细胞？
- The Laminar Wash™ plate can hold up to 10 million cells and up to 70 µl of reagents. The optimum performance in the 100,000 – 3,000,000 cells/well and the reagent volume can be increased to 150 µl by using an adaptor.
- 层流洗涤板能否在不同温度下进行孵育？
- Yes. The samples in the Laminar Wash™ plates can be incubated in a range of 2˚C -80˚C.
- 我们可以设定洗涤后的剩余量吗？
- No. The leftover volume per well is always 25 µl.
