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即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化

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Videos & Webinars跳过离心法,提高细胞回收率
跳过离心法,提高细胞回收率


描述

        层流洗涤技术提供了一种无离心机的样品制备方法,它温和且持续地洗涤样品中的细胞。通过消除离心,样品制备使细胞不再受到机械压力。带来了更好的细胞保留,并产生更高质量的数据。

        在网络研讨会的第一部分,Curiox的应用经理Chyan Ying Ke博士全面介绍了层流洗涤技术的工作原理和优点,请看层流洗涤技术是如何实现的。

网络研讨会的介绍部分所涵盖的关键点

● 细胞样品制备过程中离心所带来的挑战 (1:03)

● 层流洗涤技术如何工作 (2:42)

● 使用层流洗涤进行无离心机样品制备的好处可应用于各种应用 (5:20)

● 层流洗涤产品的特点 (6:36)

        网络研讨会的下一个环节是由来自查尔斯河实验室的Christoph Eberle博士主讲,他在肿瘤模型特征的流式细胞仪免疫分型方面具有专长。

         Eberle博士分享了宝贵的经验和数据,介绍了适应层流洗涤的无离心机样品制备如何在改善数据质量和整体工作流程方面使其工作受益。

Eberle博士提出的关键点

● TIL免疫分型的离心造成的样品制备和下游数据质量问题(13:55)

● 一个与层流洗涤技术相结合的通用细胞洗涤和染色方案的案例(16:01)

● 根据并排比较,使用层流洗涤技术处理的肿瘤和脾脏样本比人工处理的表现更好 (20:30)

o  无论起始细胞数如何,CV值更低,存活细胞更多

o   由于较高的细胞保留率和较少的碎片存在,染色结果得到改善

o   工作流程的改进,例如使用层流洗涤可节省时间和试剂

● 层流洗涤可用于处理小体积的血液样本,同时保留其优点 (27:32)

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