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即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化

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新闻 科学研究top10的分子结构作用来推断单细胞测序作用
科学研究top10的分子结构作用来推断单细胞测序作用
  因为绿色植物marker基因评定尚不够成熟,因此为了能认证top10marker基因的稳定性,实施了下列2种认证方式根据科学研究top10的分子结构作用来推断单细胞测序作用,搭建了bag6和bzip6的突变株,发觉bag6突变株表层气孔指数值提升,推断BAG6参加管控气孔的发育,从而推断Cluster9是一群参加管控气孔的发育的细胞。

  以下属于第二种认证top10marker基因的稳定性的办法:

  2015年,Adrian那篇文章要用流式的把拟南芥嫩叶划分成几种细胞,随后回收利用这种细胞去做测序,发布了测序数据来给大家用。本文就是将Figure2A中top10基因挑出,看看这些基因在人家的哪一个单细胞测序种类的信息集中化表现了。实质上就是将Figure2A中top10的marker基因,投射 到了两篇文章中,看看这些基因在这里两篇文章中是不是也在同一个细胞种类在表达。

  世界上本无marker基因,top10基因用的人多了,也就变成了marker基因。举例说明,PC(平扁外皮细胞)他没特定标示基因,本文把评定过的PC高表达基因和人家数据核对,核对到40%,也进一步能够表明细胞种类检测的靠谱水平。

  为了能发觉参加调整气孔家谱图细胞初期发育的潜在性TF,统计了在各个细胞种类上显示高表达的TFs。unknown(u.k.)_1中TFs数量较多,MPC_5中TFs数量至少。根据文献调研作者后界定了9种细胞种类,及其此外二种不能用已知标识基因来决定的单细胞测序种类。

  MMC,LM,EM和GMC都为气孔的发育早期关键细胞。剖析结果显示ATML1.BPC1.BPC6.SCRM,PIF5.andWRKY33有可能是管控MMCs、EMs、LMs和GMCs中靶基因的表达的关键转录因子。

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