即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化
以下属于第二种认证top10marker基因的稳定性的办法:
2015年,Adrian那篇文章要用流式的把拟南芥嫩叶划分成几种细胞,随后回收利用这种细胞去做测序,发布了测序数据来给大家用。本文就是将Figure2A中top10基因挑出,看看这些基因在人家的哪一个单细胞测序种类的信息集中化表现了。实质上就是将Figure2A中top10的marker基因,投射 到了两篇文章中,看看这些基因在这里两篇文章中是不是也在同一个细胞种类在表达。
世界上本无marker基因,top10基因用的人多了,也就变成了marker基因。举例说明,PC(平扁外皮细胞)他没特定标示基因,本文把评定过的PC高表达基因和人家数据核对,核对到40%,也进一步能够表明细胞种类检测的靠谱水平。
为了能发觉参加调整气孔家谱图细胞初期发育的潜在性TF,统计了在各个细胞种类上显示高表达的TFs。unknown(u.k.)_1中TFs数量较多,MPC_5中TFs数量至少。根据文献调研作者后界定了9种细胞种类,及其此外二种不能用已知标识基因来决定的单细胞测序种类。
MMC,LM,EM和GMC都为气孔的发育早期关键细胞。剖析结果显示ATML1.BPC1.BPC6.SCRM,PIF5.andWRKY33有可能是管控MMCs、EMs、LMs和GMCs中靶基因的表达的关键转录因子。
Necessary cookies are absolutely essential for the website to function properly. These cookies ensure basic functionalities and security features of the website, anonymously.
Cookie | Duration | Description |
---|---|---|
cookielawinfo-checbox-analytics | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics". |
cookielawinfo-checbox-functional | 11 months | The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". |
cookielawinfo-checbox-others | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other. |
cookielawinfo-checkbox-necessary | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary". |
cookielawinfo-checkbox-performance | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance". |
viewed_cookie_policy | 11 months | The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data. |
Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features.
Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors.
Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.
Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. These cookies track visitors across websites and collect information to provide customized ads.
Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet.