即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化
世上没有两块同样的叶片。针对多细胞微生物而言,细胞与细胞之间有存在差异的。当然,这一差别可多可少。可是当老师的样版存有下面的图这样一个状况,组织中不一样细胞的基因和基因表达不一样,为了能检验组织中蛋白质或mRNA的表达水准,各位老师能通过Westernblot和PCR或BulkRNA-seq来达到。
但用以上方式得话,各位老师并没办法分辨这种的现象:目地蛋白质只能在10%的细胞中强表述,还是50%的细胞里中等水平表述,或是在大多数细胞中弱表述呢?
举例说明,当教师给的绿色植物样版,一个基因(gene1)可能会在分生组织中上涨了,在维护组织中下调了,可是bulkRNA测序结果发觉有什么不一样,这种有什么不一样其实是个假阴性,老师会根据单细胞测序找到这类差别,开展更加深入研究。单细胞转录组测序(scRNA-seq)技术性带来了在单细胞水平上观察基因表达出来的方式,那样可以更好的科学研究这种组织中不同类型的细胞种类。
那样我们为什么要在植物中做单细胞测序呢?我们能看到这儿展现的拟南芥及其稻谷的根机构,他所涉及到的细胞种类是有一个很大的多样性的,而一样拟南芥中,根组织和叶子机构细胞种类也是不一样的,同一机构不一样细胞种类间也具有不同类型的基因表述方式,这种多样性我们都是可以利用单细胞测序技术进行深入分析的。
植物中单细胞测序实践应用
伴随着单细胞测序技术性的创新,单细胞测序的年代早已来临。其技术性早已很多发表于CELL、Nature、Science等高品质刊物中,在其中大部分都集中在动物和人层面。近些年,单细胞测序在绿色植物方位的应用还在逐渐增加,涉及到的种群包含拟南芥、稻谷,及其苞米。
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