即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化
AUTO1000是一种交钥匙、全自动的解决方案,用于制备用于流式或大规模细胞术的基于细胞的样本,具有直观的用户界面,可实现简单的分析构建。在ASGCT 2021上,Curiox Biosystems 自动化科学家Ann Wang展示了如何使用图形用户界面在 Laminar Wash AUTO1000 上设计用于流式细胞术的定制染色分析。请查看我们的其他 AUTO1000 视频资源,包括标准染色协议以及日常操作和维护。
使用 AUTO1000 的直观用户界面,定义如何从头到尾运行您的协议:
• 首先,使用Run Control选择正确的运行方法 (00:31),然后选择执行日常维护协议或使用Launcher (00:47)运行分析
• 运行选择使用户可以配置最多 2 个板的新运行,或加载预先保存的运行参数 (01:04)
• 使用“循环”(01:23)在Assay Build中构建协议。在此示例中,Ann 使用 4 个循环来执行细胞外和细胞内染色方案:
○ 循环 1:将样品转移到层流洗涤板上 (01:57)
○ 循环 2:转移细胞外染色抗体组 (03:44),包括指定使用哪个组处理哪些样本 (04:05)
○ 循环 3:使用缓冲液转移准备细胞进行细胞内染色 (05:07)
○ 循环 4:转移细胞内染色抗体组 (06:36)
• 可选择将样品转移到输出板以供以后使用,或直接在流式细胞仪上采集 (07:50)
• 板布局 图显示样品和重复的位置,以及抗体组处理的类型 (08:19)
• 选择保存运行参数以供将来使用 (09:03)
• 甲板装载图用作检查清单,以确保正确装载所有试剂和消耗品 (09:12)
• AUTO1000 系统生成:
○ 一个跟踪文件,记录运行期间的所有事件以确保可追溯性 (10:01)
○ 跟踪所有移液量的映射文件(10:31)
请联系我们以了解 AUTO1000 是否是自动化流式细胞术工作流程的正确解决方案。
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